General Science

Biotechnology, Genetics & Gene Editing

DNA/RNA structure, Recombinant DNA, CRISPR-Cas9, Stem Cells, and GM Crops.

Target: EPFO APFC 2026β€’Topic-Level RAG Context Activeβ€’Model: Nemotron 3.5 Lightning 30B / 120B

Topic Overview

DNA/RNA structure, Recombinant DNA, CRISPR-Cas9, Stem Cells, and GM Crops.

Key Syllabus Concepts

  • DNA Replication, Transcription (DNA->RNA), Translation (RNA->Protein)
  • CRISPR-Cas9 Gene Editing technology (Guide RNA, Cas9 endonuclease endonuclease)
  • Stem Cell Therapy: Pluripotent vs Multipotent vs Induced Pluripotent Stem Cells (iPSCs)
  • Genetically Modified (GM) Crops in India (Bt Cotton, DMH-11 Mustard, GEAC role)

High-Yield Important Points & Rules

  • πŸ’‘ GEAC (Genetic Engineering Appraisal Committee) functions under Ministry of Environment, Forest and Climate Change (MoEFCC).
  • πŸ’‘ CRISPR-Cas9 acts as molecular scissors to slice and edit target DNA sequences.

Historical UPSC EPFO PYQ Relevance

3 Matched PYQs
2025β€’EPFO APFCβ€’General Ability Testβ€’ Q.24
Full Question
Which of the following statements regarding the Freedom Struggle from 1920 to 1935 is/are correct?
1
In Bihar and Bengal, the Non-Cooperation Movement owed its effectiveness to the participation of peasants.
2
In Bihar they were organised against the planters under the banner of Kisan Sabha.
3
In Midnapore in Bengal, Mahishya peasants rallied against the taxes of the Union Board under the leadership of Birendranath Sasmal.
2023β€’EPFO EO/AOβ€’General Ability Testβ€’ Q.51
Full Question
Consider the following statements about DNA. Which of the statements given above is/are correct?
1
DNA refers to Deoxyribonucleic Acid.
2
It is located in the ribosomes.
3
It is composed of Ribonucleic Acid.
4
It can make a copy of itself.
2016β€’EPFO APFCβ€’General Studiesβ€’ Q.64
Full Question
DNA fingerprinting is a technique used for the detection of

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